Application Notes & Publications

Application Notes

NEW! Label-free Kinetic Analysis of Membrane Protein Interactions Using Biolayer Interferometry (BLI)

Successful production of antibodies against functional GPCR : Case study using functional CXCR4-containing liposomes

Validation of the activity of GPCRs using SPRI: case study using functional CxCR4


Synthelis Publications

Therapeutic effects of proteoliposomes on X-linked chronic granulomatous disease: proof of concept using macrophages differentiated from patient-specific induced pluripotent stem cells Julie Brault, Guillaume Vaganay, Aline Le Roy, Jean-Luc Lenormand, Sandra Cortès, Marie José Stasia. 2017.

Anti-Tumor Effects of Bak-Proteoliposomes against Glioblastoma Lavinia, Liguori, Fabio Pastorino , Xavier Rousset, Silvia Alfano, Sandra Cortes, Laura Emionite, Antonio Daga, Mirco Ponzoni and Jean-Luc Lenormand. 2015. 

Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system. Soranzo T, Cortès S, Gilde F, Kreir M, Picart C, Lenormand JL. Protein Expr Purif. 2015 

Recombinant Nox4 cytosolic domain produced by a cell or cell-free base systems exhibits constitutive diaphorase activity. 
Nguyen MV, Zhang L, Lhomme S, Mouz N, Lenormand JL, Lardy B, Morel F.Biochem Biophys Res Commun. 2012  16;419(3):453-8.

Crystallization of the membrane protein hVDAC1 produced in cell-free system. Deniaud A, Liguori L, Blesneac I, Lenormand JL, Pebay-Peyroula E
Biochim Biophys Acta. 2010 -1798(8):1540-6. 

Single-step production of functional OEP24 proteoliposomes. Liguori L, Blesneac I, Madern D, Vivaudou M, Lenormand JL. Protein Expr Purif. 2010 – 69,1 (106-111). 

Production of recombinant proteoliposomes for therapeutic uses. Liguori L. and Lenormand JL. Methods in Enzymology, 2009 – 465 (209-223).

A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: from cDNA to functional assay. Liguori L, Marques B, Lenormand JL. Curr Protoc Protein Sci. 2008. Nov;Chapter 5:Unit 5.22.

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. Liguori L, Marques B, Villegas-Mendez A, Rothe R, Lenormand JL. J Control Release. 2008. 20;126(3):217-27.

Liposome-mediated cellular delivery of active gp91(phox). Marques B, Liguori L, Paclet MH, Villegas-Mendéz A, Rothe R, Morel F, Lenormand JL. PLoS One. 2007. 12;2(9):e856. PDF here.

Production of membrane proteins using cell-free expression systems. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL. Expert Rev Proteomics. 2007. 4(1):79-90. Review.

Cell-free Publications

Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method. Ando M, Akiyama M, Okuno D, Hirano M, Ide T, Sawada S, Sasaki T, Akiyoshi K. Biomater Sci 2015

They talked about us 

Industrie Pharma N°89 Juillet Août 2015 

La gazette du laboratoire juillet 2016